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cfx96 real time pcr detection system  (Bio-Rad)


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    Structured Review

    Bio-Rad cfx96 real time pcr detection system
    Cfx96 Real Time Pcr Detection System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 28023 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cfx96+real+time+pcr/CFX96+Touch+Real-Time+PCR+Detection+System+with+Starter+Package/pmc13637308-144-5-10
    Average 96 stars, based on 28023 article reviews
    cfx96 real time pcr detection system - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Quantitative RT-PCR:

    Article Title: Comparative analysis of NF-Y transcription factors in four orchids with expression insights from Gastrodia elata
    Article Snippet: .. The qRT-PCR was performed using gene-specific primers on CFX96 Real-Time PCR (Bio-Rad, USA) with the ChamQ Blue Universal SYBR qPCR Master Mix (Nanjing Vazyme Biotech Co., Ltd., Nanjing, China), using the thermal cycling conditions of 95 °C for 30s followed by 40 cycles of 95 °C for 10s and 60 °C for 30s, and the GelActin (Gel003815) gene was used as an endogenous control. ..

    Real-time Polymerase Chain Reaction:

    Article Title: Comparative analysis of NF-Y transcription factors in four orchids with expression insights from Gastrodia elata
    Article Snippet: .. The qRT-PCR was performed using gene-specific primers on CFX96 Real-Time PCR (Bio-Rad, USA) with the ChamQ Blue Universal SYBR qPCR Master Mix (Nanjing Vazyme Biotech Co., Ltd., Nanjing, China), using the thermal cycling conditions of 95 °C for 30s followed by 40 cycles of 95 °C for 10s and 60 °C for 30s, and the GelActin (Gel003815) gene was used as an endogenous control. ..

    Article Title: Eicosapentaenoic acid enhances intestinal stem cell-mediated colonic epithelial regeneration by activating the LSD1-WNT signaling pathway
    Article Snippet: The extraction utilized the TRIzol reagent (Invitrogen), and the subsequent cDNA synthesis was performed using M−MLV (H-) Reverse Transcriptase and oligo (dT) primer (Vazyme, No. R021-01). .. The cDNA served as the template for quantitative real-time PCR, which was conducted using PowerUpTM SYBRTM Green (Applied Biosystems, No. A25741) on an CFX96 Real-Time PCR (Bio-Rad). ..

    Article Title: Synchronized Latency Reversal and Immune Clearance by a Multifunctional Fusion Protein Enables HIV-1 Reservoir Reduction
    Article Snippet: .. The probe (FAM-AAAATTCGGTTAAGGCCAGGGGGAAAGAA-QSY7) was ordered from Sangon Biotech and the reactions were set up following the manufacturer’s guidelines and were run on the CFX96 Real-Time PCR (BioRad). ..

    Article Title: Genome-wide analysis of terpene synthase family in peanut ( Arachis hypogaea L.) explores the potential cross-talk in terpenoid biosynthesis
    Article Snippet: Subsequently, the extracted RNA was reverse-transcribed to cDNA using a dedicated kit (Vazyme). qRT-PCR was conducted using 10-μL reaction mixtures containing 0.1 μM of each primer and a 1× SYBR green PCR master mix (also from Vazyme). .. Reactions were performed using a CFX96 real-time PCR system(Bio-Rad), under the following thermal cycling conditions: an initial denaturation at 95°C for 3 min, followed by 40 cycles at 95°C for 10 s and 55°C for 30 s. For each of the designated time points, we analyzed triplicate technical replicates for each of three biological replicates. ..

    Article Title: GSTT1 promotes stemness and FGFR inhibitor sensitivity in pancreatic cancer through regulation of CD133 (PROM1).
    Article Snippet: Pancreatic ductal adenocarcinoma (PDA) is among the deadliest malignancies, driven by metastatic progression and profound cellular heterogeneity.. We previously identified glutathione S-transferase theta 1 (GSTT1) as a regulator of a slow-cycling, highly metastatic tumor cell population, suggesting that GSTT1High cells may possess stem-like properties.. Here, we define the functional and molecular features of this subpopulation in metastatic PDA.

    Control:

    Article Title: Comparative analysis of NF-Y transcription factors in four orchids with expression insights from Gastrodia elata
    Article Snippet: .. The qRT-PCR was performed using gene-specific primers on CFX96 Real-Time PCR (Bio-Rad, USA) with the ChamQ Blue Universal SYBR qPCR Master Mix (Nanjing Vazyme Biotech Co., Ltd., Nanjing, China), using the thermal cycling conditions of 95 °C for 30s followed by 40 cycles of 95 °C for 10s and 60 °C for 30s, and the GelActin (Gel003815) gene was used as an endogenous control. ..

    SYBR Green Assay:

    Article Title: GSTT1 promotes stemness and FGFR inhibitor sensitivity in pancreatic cancer through regulation of CD133 (PROM1).
    Article Snippet: Pancreatic ductal adenocarcinoma (PDA) is among the deadliest malignancies, driven by metastatic progression and profound cellular heterogeneity.. We previously identified glutathione S-transferase theta 1 (GSTT1) as a regulator of a slow-cycling, highly metastatic tumor cell population, suggesting that GSTT1High cells may possess stem-like properties.. Here, we define the functional and molecular features of this subpopulation in metastatic PDA.

    Real-time Monitoring:

    Article Title: GSTT1 promotes stemness and FGFR inhibitor sensitivity in pancreatic cancer through regulation of CD133 (PROM1).
    Article Snippet: Pancreatic ductal adenocarcinoma (PDA) is among the deadliest malignancies, driven by metastatic progression and profound cellular heterogeneity.. We previously identified glutathione S-transferase theta 1 (GSTT1) as a regulator of a slow-cycling, highly metastatic tumor cell population, suggesting that GSTT1High cells may possess stem-like properties.. Here, we define the functional and molecular features of this subpopulation in metastatic PDA.

    Polymerase Chain Reaction:

    Article Title: GSTT1 promotes stemness and FGFR inhibitor sensitivity in pancreatic cancer through regulation of CD133 (PROM1).
    Article Snippet: Pancreatic ductal adenocarcinoma (PDA) is among the deadliest malignancies, driven by metastatic progression and profound cellular heterogeneity.. We previously identified glutathione S-transferase theta 1 (GSTT1) as a regulator of a slow-cycling, highly metastatic tumor cell population, suggesting that GSTT1High cells may possess stem-like properties.. Here, we define the functional and molecular features of this subpopulation in metastatic PDA.

    Amplification:

    Article Title: GSTT1 promotes stemness and FGFR inhibitor sensitivity in pancreatic cancer through regulation of CD133 (PROM1).
    Article Snippet: Pancreatic ductal adenocarcinoma (PDA) is among the deadliest malignancies, driven by metastatic progression and profound cellular heterogeneity.. We previously identified glutathione S-transferase theta 1 (GSTT1) as a regulator of a slow-cycling, highly metastatic tumor cell population, suggesting that GSTT1High cells may possess stem-like properties.. Here, we define the functional and molecular features of this subpopulation in metastatic PDA.

    other:

    Article Title: EB1010, a putative Christensenellaceae-derived microbial peptide, exhibits anti-inflammatory activity and therapeutic potential in ulcerative colitis
    Article Snippet: Quantitative PCR was performed using Takyon No ROX SYBR 2× Master Mix blue dTTP (Eurogentec, Seraing, Belgium).

    Article Title: EB1010, a putative Christensenellaceae-derived microbial peptide, exhibits anti-inflammatory activity and therapeutic potential in ulcerative colitis.
    Article Snippet: Quantitative PCR was performed using Takyon No ROX SYBR 2× Master Mix blue dTTP (Eurogentec, Seraing, Belgium).

    Article Title: Plasma 1 H NMR-based metabolomic analysis reveals the lipid regulating effect of novel carboxamide derivatives in hyperlipidemic rats
    Article Snippet: cDNA synthesis was done using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, USA) and the obtained cDNA samples were stored at -20°.



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